Correlation between riboflavin carrier protein induction and its mRNA activity in estrogen stimulated chicken liver and oviduct

Poly A enriched RNA from either liver or oviduct of estradiol-17β treated immature chicks supported [3H]-leucine incorporation into immunoprecipitable riboflavin carrier protein in a dose-dependent manner when translated in the rabbit reticulocyte lysate system. Primary translation product of riboflavin carrier protein had a molecular weight of 38,000 which on incubation with a stripped hepatic microsomal preparation was processed to a product with a size comparable to native riboflavin carrier protein. Poly A enriched RNA from both the liver and the oviduct of estrogen-treated birds stimulated [3H]-leucine incorporation into riboflavin carrier protein and this was 2-3 fold higher during secondary stimulation vis-a-vis primary stimulation with the steroid. Poly A enriched RNA from the liver of progesteronetreated birds during secondary stimulation did not support riboflavin carrier protein synthesis. In contrast, poly A enriched RNA from the oviduct of the birds treated with progesterone during secondary (but not primary) stimulation did exhibit riboflavin carrier protein-mRNA activity which was comparable to that stimulated by estradiol-17β

Potential of Probiotic Recombinant Microbial Enzymes: Overview of Expression, Purification, Characterization and Its Application in Various Diseases

Probiotics, beneficial bacteria with significant therapeutic potential, have gained attention for addressing a range of health issues, including gastrointestinal disorders, immune deficiencies, and mental health concerns. This article delves into the expression and purification of recombinant microbial enzymes to harness the therapeutic potential of probiotics. Probiotics offer promise in promoting overall well-being and preventing diseases through their ability to modulate immune responses and enhance gut health. They have proven effective in alleviating symptoms related to conditions like irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and antibiotic-associated diarrhea. These live microorganisms, when administered in sufficient quantities, restore gut flora balance and modulate immune responses. By engineering probiotics to produce therapeutic enzymes that aid digestion or regulate gut microbiota, they can significantly contribute to digestive health and mitigate IBS and IBD symptoms. The expression and purification of recombinant microbial enzymes play a crucial role in enhancing probiotics' therapeutic capabilities, enabling tailored treatments for various health challenges. In summary, probiotics represent a promising frontier in therapeutic microbiology and biotechnology. Their multifaceted benefits, when combined with recombinant microbial enzyme expression and purification, hold immense potential for advancing the field of therapeutic probiotics and improving human health

The Versatility of Perforator-Based Propeller Flap for Reconstruction of Distal Leg and Ankle Defects

Introduction. Soft tissue coverage of distal leg and ankle region represents a challenge and such defect usually requires a free flap. However, this may lead to considerable donor site morbidity, is time consuming, and needs facility of microsurgery. With the introduction of perforator flap, management of small- and medium-size defects of distal leg and ankle region is convenient, less time consuming, and with minimal donor site morbidity. When local perforator flap is designed as propeller and rotated to 180 degree, donor site is closed primarily and increases reach of flap, thus increasing versatility. Material and Methods. From June 2008 to May 2011, 20 patients were treated with perforator-based propeller flap for distal leg and ankle defects. Flap was based on single perforator of posterior tibial and peroneal artery rotated to 180 degrees. Defect size was from 4 cm × 3.5 cm to 7 cm × 5 cm. Results. One patient developed partial flap necrosis, which was managed with skin grafting. Two patients developed venous congestion, which subsided spontaneously without complications. Small wound dehiscence was present in one patient. Donor site was closed primarily in all patients. Rest of the flaps survived well with good aesthetic results. Conclusion. The perforator-based propeller flap for distal leg and ankle defects is a good option. This flap design is safe and reliable in achieving goals of reconstruction. The technique is convenient, less time consuming, and with minimal donor site morbidity. It provides aesthetically good result

Fully Invariant and Characteristic Interval-Valued Intuitionistic Fuzzy Dual Ideals of BF-algebras

The notion of interval-valued intuitionistic fuzzy sets was first introduced by Atanassov and Gargov as a generalization of both interval-valued fuzzy sets and intuitionistic fuzzy sets. Satyanarayana et. al., applied the concept of interval-valued intuitionistic fuzzy ideals and interval-valued intuitionistic fuzzy dual ideals to BF-algebras. In this paper, we introduce the notion of fully invariant and characteristic interval-valued intuitionistic fuzzy dual ideals of BF-algebras and investigate some of its properties

Comparison of active versus expectant management on fetomaternal outcome in patients with placenta previa

Background: Placenta previa is one of the leading cause (31%) of obstetric hemorrhage. It accounts for significant maternal and perinatal morbidity and mortality. The objective of the present investigation was to compare the effect of active management versus expectant management on maternal and fetal outcome in patients with placenta previa.Methods: This randomized prospective cohort study was conducted on 100 Patients of 32 weeks to 36 weeks of gestation with diagnosis of Placenta previa. Selected patients were randomly divided into Group A- Active management and Group B - Expectant management.Results: In both the groups, majority of women were in the age group of 26-30 years and were multigravidas. The need for blood transfusion and the mode of delivery were similar in both the groups. Group A had higher incidence of PPH (22% vs 10%) and peripartum hysterectomy (18% vs 2%) when compared to group B. The maternal deaths were more in group A (4% vs 2%). Majority of the babies born to group A mothers had a low Apgar, birth weight below 2 kgs and greater NICU admission. The perinatal deaths were more in group A (16% vs 2%) and the difference was statistically significant.Conclusions: The expectant management protocol was concluded to be a better mode of management protocol in patients with placenta praevia, who are either asymptomatic or with mild to moderate bleeding

Cervical lymphadenopathy – an unusual presentation of carcinoma of the cervix: a case report

<p>Abstract</p> <p>Introduction</p> <p>The clinical presentation of carcinoma of the cervix as cervical lymphadenopathy has not been described before. We report a case of this unusual manifestation of cervical cancer.</p> <p>Case presentation</p> <p>A 51-year-old woman presented to our Head and Neck department with cervical lymphadenopathy. A positron emission tomography scan revealed the primary tumour to be in the cervix and a cervical biopsy confirmed carcinoma of the cervix.</p> <p>Conclusion</p> <p>Recurrences of carcinoma of the cervix presenting as lymphadenopathy have been described before but this is the first time a clinical presentation of carcinoma of the cervix as cervical lymphadenopathy has been described. Although metastasis from the cervix to the cervical lymph nodes is rare, this can be explained by outlining the drainage of the lymphatic system from the cervix.</p

Effect of maternal obesity on fetal iron transfer

INTRODUCTION: WHO defines obesity or overweight as abnormal fat accumulation that presents a risk to life. According to National survey of family health report overall prevalence of overweight among women of 20-44 years was 24.4%and 23% were obese. BACKGROUND OF STUDY: According to primary literature, obesity increases inflammatory mediator CRP and hepcidin. Hepcidin binds to ferroportin and inactivates it and prevents the iron transfer to the fetus. Decreased iron transfer may result in neurodevelopmental impairment to the fetus. AIM OF THE STUDY: 1. To demonstrate the effect of maternal obesity and fetal iron transfer by taking normal pregnant women in three groups based on their BMI. 2. To correlate the effect of inflammatory mediators in early pregnancy on cord blood iron status. 3. To sensitize the women to reduce their weight before contemplating the next pregnancy. MATERIALS AND METHODS: In first trimester during first visit after confirmation of pregnancy, the pregnant women are divided into three groups based on BMI after obtaining their consent. Group 1: Normal pregnant women with BMI (18-24.9kg/sq.m). Group 2: Overweight pregnant women with BMI (25-29.9kg/sq.m). Group 3: Obese pregnant women with BMI (>30kg/sq.m). Sample size: 90 (30 pregnant women in each group). Study period: June 2013-May 2014. (12 Months). Study design: Prospective Cross Sectional Cohort Study. These pregnant women were followed throughout the pregnancy to look for any complications inherent to pregnancy which would be recorded and they were excluded from the study. They were followed throughout the pregnancy. In second trimester, maternal CRP investigation was done. At the time of delivery the cord blood was collected and it is subjected for cord blood iron profile which includes serum iron, transferrin saturation and serum ferritin and cord blood CRP. PRIMARY OUTCOME: The relationship between maternal obesity and cord blood iron profile. SECONDARY OUTCOME: To correlate the effect of inflammatory mediators like CRP in early pregnancy on fetal iron transfer

Correlation of Chemical Properties of A-Niti Wires with Mechanical Properties: An Invitro study

BACKGROUND: Nickel titanium was introduced by Anderson in the year 1971. Due to their characteristics of low stiffness, superelesticity, and high reversibility, it is one of the most essential archwires in orthodontic biomechanics. It is highly effective in the malocclusion with severe crowding that requires large deflections with light continuous forces. The chemical composition and mechanical properties varies in the same dimension of archwires from different manufacturers due to alloying composition and processing methods. AIM OF THE STUDY: To correlate the chemical properties with mechanical properties of four different commercially available A-NiTi wires. MATERIALS AND METHODS: The study done with four commercial groups (G and H, Dentaurum, Essential dental products and American orthodontics) of A-NiTi rectangular archwires of size (0.017 X 0.025), the four mechanical properties microhardness tested by Vickers microhardness test, the microstructure observed under 200X magnification, the ultimate tensile strength and percentage elongation done using universal tensile tester machine. Chemical analyzed using X-ray fluorescence energy dispersion automated spectroscopy method. RESULTS: From the finding observed in this present study, it can be concluded that microhardness tests showed that statistically significant differences (p=0.000) were present between each among the four groups according to Bonferroni post hoc comparison. The microstructure observed that fine grains was present in group1, 2 (G and H and Dentaurum) and coarse grains in group 3, 4 (essential dental products, American orthodontics). One way ANOVA test showed statistically significant differences present between each group with the highest values of UTS and least values of percentage elongation in group-2 (Dentaurum). Bonferroni pair wise comparison to compare the percentage elongation among the four groups showed that there was statistically significant difference (P=0.000) observed only in group-1(G and H), when compared each other individual groups. Group-1 had the highest percentage elongation value. Statistically significant variation in chemical trace elements and zinc among the four groups, with highest zinc content was found in group-1 G and H (0.24%), followed by group -2 (0.23%), group-3 (0.11%) and group 4 (0.10%). The trace elements were the highest in both group 3 and group 4 which had about 0.26%, followed by group-1 G and H which consisted of 0.23% and the least in group-2 Dentaurum with 0.16% of total net composition. person correlation coefficient done to correlate the mechanical properties with chemical analysis and concluded that strong correlation with (r value higher than 0.5 and closer to 1) present between microhardness, UTS and percentage due to zinc and trace elements in all the four groups NiTi. CONCLUSION: The microhardness value was the highest in the group-2(Dentaurum) showed that increase in microhardness can be correlated with lesser amount of trace elements present. The ultimate tensile strength (UTS) associated with group-1(G and H) was concluded that decrease in UTS can be correlated with increase in zinc content. The percentage elongation was the highest in group-1, (G and H) and showed due to presence with increase in zinc content. There was inverse proportionality was present in UTS and percentage elongation was observed in all the four groups and was concluded that NiTi were highly flexible

Expectation of forward-backward rapidity correlations in p+pp+p collisions at the LHC energies

Forward-backward correlation strength ($b$) as a function of pesudorapidity intervals for experimental data from $p+\bar{p}$ non-singly diffractive collisions are compared to PYTHIA and PHOJET model calculations. The correlations are discussed as a function of rapidity window ($\Delta \eta$) symmetric about the central rapidity as well as rapidity window separated by a gap ($\eta_{gap}$) between forward and backward regions. While the correlations are observed to be independent of $\Delta \eta$, it is found to decrease with increase in $\eta_{gap}$. This reflects the role of short range correlations and justifies the use of $\eta_{gap}$ to obtain the accurate information about the physics of interest, the long range correlations. The experimental $b$ value shows a linear dependence on $\ln \sqrt{s}$ with the maximum value of unity being reached at $\sqrt{s}$ = 16 TeV, beyond the top LHC energy. However calculations from the PYTHIA and PHOJET models indicate a deviation from linear dependence on $\ln \sqrt{s}$ and saturation in the $b$ values being reached beyond $\sqrt{s}$ = 1.8 TeV. Such a saturation in correlation values could have interesting physical interpretations related to clan structures in particle production. Strong forward-backward correlations are associated with cluster production in the collisions. The average number of charged particles to which the clusters fragments, called the cluster size, are found to also increase linearly with $\ln \sqrt{s}$ for both data and the models studied. The rate of increase in cluster size vs. $\ln \sqrt{s}$ from models studied are larger compared to those from the data and higher for PHOJET compared to PYTHIA. Our study indicates that the forward-backward measurements will provide a clear distinguishing observable for the models studied at LHC energies.Comment: 15 pages, 14 Figures, accepted for publication in International Journal of Modern Physics